Phosphate buffered Saline (PBS)
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Phosphate buffered Saline (PBS)
Phosphate buffered saline (PBS) is a commonly used solution in the cell culture manufacturing process and in the early stages of process development. It helps to maintain the pH of cell culture media by acting as a buffer.
Phosphate buffered Saline (PBS)
ერთეულის #: 86986919

Phosphate buffered Saline (PBS)

ერთეულის #: 86986919

GEL 112

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Phosphate buffered saline (PBS) is a commonly used solution in the cell culture manufacturing process and in the early stages of process development. It helps to maintain the pH of cell culture media by acting as a buffer.
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What Stands Out

pH Stability
PBS maintains a stable pH environment, crucial for biological experiments, ensuring accurate and reliable results across various applications, including cell culture and molecular biology.
Versatile Application
Ideal for diluting proteins and other biological samples, PBS is widely used in laboratories, making it an essential buffer for research across numerous scientific fields.
High Purity
Developed with high-purity ingredients, PBS is free from contaminants, ensuring high-quality results in sensitive experiments, which is essential for researchers seeking reliable data.

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Who Should Buy?

Suitable For
  • Cell Culture Researchers

    PBS provides an isotonic solution ideal for maintaining the viability of cells during culture and experimental procedures.

  • Molecular Biologists

    Used to dilute samples and wash steps, PBS is crucial for various molecular biology techniques, such as PCR and Western blotting.

  • Diagnostic Laboratories

    PBS is commonly used in diagnostics to prepare reagents, buffers, and diluents necessary for various assay protocols.

Not Suitable For
  • Animal Studies

    PBS may not provide the required nutrients for animal cell growth or maintain physiological conditions in live animal studies.

  • High Salt Experiments

    Not suitable for experiments requiring high concentrations of salts, as it may interfere with results and cellular behavior.

  • Long-Term Storage

    PBS is not equipped for long-term cell storage; alternatives like cryoprotectants should be used to preserve cellular integrity.

პროდუქტის აღწერილობა

Phosphate buffered Saline (PBS)

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მომხმარებელთა შეკითხვები და პასუხები

  • კითხვა: What is Phosphate Buffered Saline (PBS)?

    პასუხი: Phosphate Buffered Saline (PBS) is a buffered saline solution that maintains a consistent pH and osmolarity, making it ideal for biological applications. It primarily consists of sodium chloride, potassium chloride, sodium phosphate, and potassium phosphate, which create a stable environment for cells and biological materials. PBS is commonly used in cell culture, molecular biology, and as a wash solution for various lab procedures to ensure that the treated cells remain healthy and viable.
  • კითხვა: What are the main uses of PBS in laboratories?

    პასუხი: PBS is widely used in laboratories for various applications, including cell culture, immunohistochemistry, and molecular biology experiments. It helps maintain osmotic balance and pH for cells cultured in vitro while also cleaning and diluting samples. These properties make PBS essential for preparing samples for analysis, washing away unbound antibodies in immunoassays, and serving as a diluent for reagents and staining solutions.
  • კითხვა: Is PBS suitable for all types of cell cultures?

    პასუხი: PBS is generally suitable for various mammalian cell cultures, as it provides a stable environment. However, specific cell lines may have unique requirements regarding growth media that PBS alone cannot fulfill. It’s often used as a wash or dilution solution rather than a growth medium. For optimal results, it is advisable to consult the culture manuals or protocols specific to your cell line to ensure appropriate conditions.
  • კითხვა: How should PBS be stored?

    პასუხი: PBS should be stored in a cool, dry place away from direct sunlight. If prepared fresh, it’s typically stable at room temperature for a short duration. For prolonged storage, it’s best kept at 4°C. Avoid repeated freeze-thaw cycles, as they can degrade the components of the solution. Proper storage practices help maintain the integrity of the buffer, ensuring reliable performance in experimental procedures.
  • კითხვა: Can PBS be used for in vivo studies?

    პასუხი: While PBS is primarily used in vitro for cell culture and laboratory applications, it can also be utilized in in vivo studies as a saline solution to maintain fluid and electrolyte balance. However, in such cases, it is essential to consider the specific requirements of the study and how PBS interacts with biological systems to ensure the validity of the results.
  • კითხვა: What precautions should I take when handling PBS?

    პასუხი: When handling PBS, wear appropriate personal protective equipment (PPE) such as gloves and safety glasses to prevent any potential skin or eye irritation. Follow standard laboratory safety protocols to minimize contamination. Additionally, always use sterile techniques to prepare solutions, especially when working with cell cultures, to prevent unwanted contamination and ensure the accuracy of your experiments.
  • კითხვა: How is PBS prepared in the lab?

    პასუხი: PBS can be prepared by dissolving the appropriate amounts of sodium chloride, potassium chloride, and phosphate buffers in distilled water. The exact recipe may vary slightly depending on the desired pH and osmolarity. Alternatively, PBS is also available in ready-to-use formulations, which can save time in the lab. Following proper preparation protocols is important to maintain the buffer's effectiveness for sensitive applications.
  • კითხვა: What is the pH range of PBS, and why is it important?

    პასუხი: PBS typically has a pH range of about 7.2 to 7.4, which is crucial for maintaining cellular functions during experimental procedures. A stable pH ensures optimal conditions for enzymatic reactions, protein stability, and overall cell health. Deviations from this pH can negatively impact biological experiments, so the buffering capacity of PBS helps to prevent such fluctuations, making it a reliable choice for laboratory use.
  • კითხვა: Can I use PBS for washing biological samples?

    პასუხი: Yes, PBS is commonly used for washing biological samples, particularly in immunoassays and cell culture. Its isotonic and buffered nature helps to maintain cell integrity while effectively removing unbound substances, such as antibodies. This cleaning step is essential to minimize background noise in assays and enhance the accuracy of results, making PBS an invaluable component of many laboratory protocols.
  • კითხვა: Where can I buy Phosphate Buffered Saline (PBS) in Georgia?

    პასუხი: You can buy Phosphate Buffered Saline (PBS) in Georgia from Ubuy. They offer a range of PBS products suitable for laboratory use, ensuring you have access to quality solutions for your scientific needs. Ubuy provides a reliable platform where you can find the specific formulations you require for your research or experimentation.

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  • შეზღუდვები: გთხოვთ, გაითვალისწინოთ, რომ საზღვარგარეთ გაგზავნილი პროდუქტებისთვის მწარმოებლის გარანტია შეიძლება არ იყოს მოქმედი; მწარმოებლის მომსახურების პარამეტრები შეიძლება არ იყოს ხელმისაწვდომი; პროდუქტის ინსტრუქციები და უსაფრთხოების გაფრთხილებები შეიძლება არ იყოს დანიშნულების ქვეყნის ენაზე; პროდუქტები (და მასთან დაკავშირებული მასალები) შეიძლება არ იყოს შემუშავებული დანიშნულების ქვეყნის სტანდარტების, სპეციფიკაციებისა და მარკირების მოთხოვნების შესაბამისად; და პროდუქტები შეიძლება არ შეესაბამებოდეს დანიშნულების ქვეყნის ძაბვისა და სხვა ელექტრო სტანდარტებს (საჭიროების შემთხვევაში საჭიროა ადაპტერი ან გადამყვანი). მიმღები პასუხისმგებელია უზრუნველყოს, რომ პროდუქტი კანონიერად შემოიტანოს დანიშნულების ქვეყანაში. Ubuy-ს ან მისი აფილირებული პირების მეშვეობით შეკვეთისას მიმღები არის რეგისტრირებული იმპორტიორი და მან უნდა გაითვალისწინოს დანიშნულების ქვეყნის ყველა კანონი და დებულება.
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მახასიათებლები და უპირატესობები

  • PBS is a vital solution in cell culture manufacturing
  • Maintains pH of cell culture media by acting as a buffer
  • Versatile usage: washing cells, transporting cells, diluting cells for counting, and preparing reagents
  • Formulated without calcium and magnesium
  • Produced in a cGMP-compliant facility with stringent quality control measures
  • Filtered through a 0.1 μm sterile filter and tested for sterility, pH, osmolality, and endotoxin

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